Airway Epithelial Cell Core

Principal Investigator(s):

Steven L. Brody, M.D. - Medicine

Status: Completed

Center(s): Center for Pediatric Pulmonary Disease

Award Mechanism: Core Large Initiative

Project Period: 7/1/2011 - 6/30/2014

Total Amount: $274,500

Collaborators: Jeffrey J. Atkinson, Thomas W. Ferkol, G. Alexander Patterson, Darrell Kotton

Project Summary:
Culture of airway epithelial cells has become an essential model for the study of lung disease and crucial for understanding the biology and therapy of asthma, respiratory infections and cystic fibrosis, all important causes of lung diseases of children. In this Core, lung cells from surgery or biopsy are cultured under specific conditions into the specialized cells that line the airways. Since being established in 2007 with CDI funding, this Core has generated over 200 cell preparations, assisted over 25 investigators, and contributed to substantial success with NIH and other funding. Dr. Steven Brody, a pulmonary physician and expert in airway epithelial cell biology, directs this Core. Collaborators include Dr. Stuart Sweet in Pediatrics, Dr. Dan Rosenbluth and Dr. Jeff Atkinson in Medicine and Dr. Alec Patterson, Chief of Cardiothoracic Surgery. Aims: - Provide high quality primary culture airway epithelial cell preparations from human and mouse to investigate biology and therapeutic strategies for lung diseases in children. - Provide consultation and training of investigators. - Develop a tissue repository of normal and diseased lung epithelial cells. - Differentiate induced pluripotent stem cells (iPSC) from patients with primary ciliary dyskinesia into airway epithelial cells as a strategy to facilitate the study of this rare disease of children. Potential impact: The Airway Epithelial Cell Core provides access to a model system that is vital for investigation of lung diseases in children. Advantages of this core include standardization of culture methods, resource sharing of valuable human cells, cost-effective utilization of reagents and supplies, enhancing the efficiency of already funded projects and attraction of new investigators.