This award will partially support the purchase and maintenance of a Nano-LC-LTQ-orbitrap. This proteomics platform will benefit CDI investigators in both basic research and clinical studies in the four Centers of the CDI.
Final Report: Several research teams funded by the Children's Discovery Institute have used these high-resolution mass spectrometric (MS) capabilities. For example, one group used the technology to study the host immune response to a variety of disease-causing organisms that appear in children's feces. By employing the high-resolution MS technology, clear groupings of specimens from children with bacterial and viral infections, as well as controls, emerged. Protein networks within each sample were identified with Ingenuity Pathways® and MetaCore® software. Some of these protein networks were elevated in all children with bacterial infections of the gastrointestinal tract, providing targets for further study. In other research, high-resolution MS helped to define and quantitate the integral membrane proteins in the E. coli bacteria, proteins that determine how the bacteria interacts with, and ultimately infects, a child's body. Such studies may give rise to new strategies for modulating interactions between bacteria and the cells of a child's gut and other similar cells in the body. Institute-funded applicstion of MS technology has contributed to a total of four collaborative publications in major scientific journals.